Exploring Porcine Precision-Cut Kidney Slices as a Model for Transplant-Related Ischemia-Reperfusion Injury
نویسندگان
چکیده
Marginal donor kidneys are more likely to develop ischemia-reperfusion injury (IRI), resulting in inferior long-term outcomes. Perfusion techniques used attenuate IRI and improve graft quality. However, machine perfusion is still its infancy, research required for optimal conditions potential repairing therapies. Experimental using porcine a great way investigate transplant-related IRI, but these experiments costly time-consuming. Therefore, an intermediate model study would be of value. We developed precision-cut kidney slice (PCKS) that resembles provides opportunities studying multiple interventions simultaneously. Porcine were procured from local slaughterhouse, exposed 30 min warm ischemia, cold preserved. Subsequently, PCKS prepared incubated under various conditions. Adenosine triphosphate (ATP) levels histological tissue integrity assessed renal viability injury. Slicing did not influence viability, remained viable up 72 h incubation with significantly increased ATP levels. Hypothermic normothermic led higher than baseline. William’s medium E supplemented Ciprofloxacin (and Amphotericin-B) provided the most beneficial condition PCKS. The can transplant IRI.
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ژورنال
عنوان ژورنال: Transplantology
سال: 2022
ISSN: ['2673-3943']
DOI: https://doi.org/10.3390/transplantology3020015